Abcam, ab258709, Human TAOK2 knockout HEK-293T cell lysate

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TAOK2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TAOK2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAOK2 a serine/threonine-protein kinase also known as TAO2 is a member of the thousand and one amino acid kinases family. It is a 130 kDa protein that participates in various cellular processes by transferring phosphate groups to serine and threonine residues on substrate proteins. TAOK2 is part of the cytoplasmic signaling pathway and shows expression in different tissues especially in the brain. It plays important roles by interacting with cellular machinery to perform its functions effectively within the cell.
Biological function summary
TAOK2 is instrumental in modulating cytoskeletal dynamics impacting processes such as neuron morphology and dendritic branching. TAOK2 associates with complexes involved in microtubule assembly influencing cell division and intracellular transport. Its activity affects neuronal connectivity impacting neural development and synaptic function which are critical in the nervous system. This makes its regulation necessary for normal brain function and development.
Pathways
TAOK2 integrates into the MAPK signaling pathway affecting cell responses to stress and cytokines. This pathway is important for initiating cellular responses to external stimuli such as changes in the environment or cellular damage. TAOK2 phosphorylates other proteins such as MEK3 MEK6 and p38 MAP kinases influencing downstream signaling cascades. It also interacts with proteins involved in the Wnt signaling pathway contributing to cell differentiation proliferation and the maintenance of stem cells.
TAOK2 has links to neurodevelopmental disorders and schizophrenia. Mutations and dysregulations in TAOK2 expression correlate with developmental abnormalities in the brain potentially leading to conditions like autism spectrum disorder. Furthermore its interaction with proteins such as DISC1 a schizophrenia-associated protein suggests a role in psychiatric disorders. TAOK2's involvement in altering neural architecture highlights its importance in maintaining neuropsychiatric health.