Abcam, ab25871, Anti-HIV1 Rev antibody [10]

Size: 100µg
Mouse Monoclonal REV antibody. Suitable for RipA, WB, ELISA and reacts with Human immunodeficiency virus, Recombinant full length protein - Human immunodeficiency virus samples. Cited in 2 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:10,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human immunodeficiency virus,
Applications:ELISA, RipA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Epitope:Epitope mapped to aa34-40

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HIV-1 Rev protein also known as Regulatory protein Rev is an essential component of the HIV-1 virus. It weighs approximately 13 kDa and plays an important role in the viral replication cycle. HIV-1 Rev is expressed in cells infected by the HIV-1 virus predominantly within the nucleus. It interacts with viral RNA elements known as the Rev Response Elements (RRE) helping in the regulation of viral mRNA during the HIV-1 life cycle. Researchers often use techniques like Western blotting to detect and study this protein due to its significant role in HIV infection.
Biological function summary
The Rev protein facilitates a critical stage in the gene expression of HIV in infected cells. It aids in the export of intron-containing viral RNA from the nucleus to the cytoplasm ensuring that these RNAs are effectively translated into viral proteins. HIV-1 Rev does not function alone; it forms part of a complex involving other cellular proteins such as exportin-1 (CRM1) that helps in its transport functions. This complex interaction allows the virus to efficiently reproduce by bypassing the normal cellular restrictions on nuclear export.
Pathways
The HIV-1 Rev protein interacts with pathways involved in viral mRNA transport and gene expression regulation. It is particularly significant in the CRM1-mediated nuclear export pathway. Through this pathway Rev is related to various proteins such as Ran GTPase which is critical for the nucleocytoplasmic transport mechanisms. These pathways are vital for the replication and assembly of new viral particles within HIV-infected cells.
The HIV-1 Rev protein is a central figure in the progression of HIV/AIDS. It controls the expression of viral genes impacting how rapidly and effectively the virus multiplies. In the context of HIV-related diseases Rev influences proteins involved in the host cell's regulatory systems including the p53 tumor suppressor protein which can affect cell cycle and apoptosis. Understanding Rev's function and interactions is important in developing treatments that target and mitigate the progression of HIV/AIDS.