Abcam, ab258735, Human TRIM16 knockout HeLa cell lysate

Size: 1Kit
TRIM16 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRIM16, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRIM16 also known as Tripartite Motif Containing 16 is a protein involved in several cellular mechanisms. It has a molecular mass of approximately 64 kDa. This protein is part of the TRIM protein family characterized by the presence of a RING finger domain one or two zinc-binding motifs called B-boxes and a coiled-coil region. TRIM16 is expressed in many tissues including the skin lungs and brain. The protein exhibits E3 ubiquitin ligase activity which tags other proteins for degradation.
Biological function summary
This protein plays a role in regulating cell differentiation and proliferation. TRIM16 is not known to form large complexes but its interactions with other proteins allow it to exert its biological effects. It interacts with factors that influence cellular growth pathways contributing to the control of cellular homeostasis. Its functional roles include modulation of specific transcription factors and involvement in the regulation of inflammation.
Pathways
TRIM16 participates in the interferon signaling pathway and the Wnt/β-catenin signaling pathway. Its involvement in the interferon signaling pathway indicates a role in immune response regulation while its activity in the Wnt/β-catenin pathway suggests a function in cellular development and differentiation. TRIM16 interacts with proteins like β-catenin and STAT1 integrating signals from different pathways to maintain normal cellular functions.
Alterations in TRIM16 expression and function associate with cancer and inflammatory diseases. In cancer TRIM16 often acts as a tumor suppressor with its underexpression linked to increased tumorigenic potential. It interacts with proteins such as p53 to carry out these functions. In the context of inflammatory diseases TRIM16 influences cytokine production closely connected with inflammation regulation and interacts with proteins like NF-kB.