Abcam, ab258741, Human TUBB2A knockout HEK-293T cell lysate

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TUBB2A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TUBB2A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TUBB2A also known as tubulin beta-2A chain is an important component of microtubule structures which are integral for cell shape intracellular transport and division. The protein has a molecular mass of approximately 49 kDa and forms the heterodimer with alpha-tubulin. These heterodimers further polymerize to create microtubules. TUBB2A expression is widespread showing high levels in neuronal tissues where microtubule dynamics play a big role in proper neuronal function and development.
Biological function summary
TUBB2A plays a central part in the polymerization and depolymerization of microtubules which are essential processes in mitosis and meiosis. As part of the tubulin superfamily TUBB2A associates closely with other tubulin isoforms to form dynamic microtubule structures. These structures undergo rapid assembly and disassembly which are critical for chromosome segregation during cell division and for intracellular transport in axons.
Pathways
TUBB2A functions within the microtubule dynamics pathway and is importantly involved in the mitotic spindle assembly ensuring accurate chromosome segregation. This protein interacts with motor proteins like kinesins and dyneins which facilitate cellular transport and mitotic processes. Additionally it participates in the neuronal signaling pathways interacting with tau and other associated proteins involved in microtubule stability and synaptic function.
TUBB2A mutations have been linked to lissencephaly a rare brain malformation characterized by a smooth cerebral surface and developmental delays. These mutations disrupt microtubule stability affecting neuronal migration. Additionally neurodegenerative diseases such as Alzheimer's have demonstrated connections to TUBB2A through interactions with tau protein which is known to form pathological aggregates in these conditions.