Abcam, ab258940, Human LAPTM4A knockout HEK-293T cell lysate

Size: 1Kit
LAPTM4A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LAPTM4A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAPTM4A also known as Lysosomal-associated transmembrane protein 4 alpha is a protein involved in intracellular trafficking. It has a molecular weight of approximately 28 kDa. LAPTM4A consists of four predicted transmembrane domains and expresses in various tissues with high levels in the spleen kidney and liver. It plays a role in endosomal and lysosomal pathways influencing cellular processes like nutrient transport and recycling.
Biological function summary
This protein contributes to cell comprehension by facilitating lysosomal function and maintaining cellular homeostasis. LAPTM4A participates in the regulation of proliferation and survival often forming a complex with other lysosomal proteins for transmitting signals related to these processes. It impacts cellular signaling pathways by interacting with certain membrane receptors and transporting them to lysosomes for degradation or recycling.
Pathways
LAPTM4A is an integral part of endocytic and vesicular transport pathways. It influences receptor-mediated endocytosis that directs cell surface proteins to lysosomes for degradation or recycling. In these pathways LAPTM4A interacts with other proteins like c-MET a receptor tyrosine kinase that plays a part in signal transduction processes linked to cell growth.
Mutations or alterations in LAPTM4A levels can connect to cancer and psychiatric disorders like schizophrenia. The protein's interaction with c-MET has identified its role in tumor progression where abnormal c-MET signaling contributes to cancerous growth. Furthermore alterations in LAPTM4A can affect neurotransmitter systems associating the protein with schizophrenia's pathophysiology.