Abcam, ab258943, Human LIX1L knockout HeLa cell lysate

Size: 1Kit
LIX1L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LIX1L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIX1L also known as Lim Homeobox Protein 1-Like is a protein encoded by the LIX1L gene with a mass of approximately 28.8 kDa. This protein is expressed in various tissues including the brain and testis indicating its role in multiple physiological contexts. LIX1L's structure suggests it could interact with DNA or proteins but its precise molecular mechanisms remain an area for further study.
Biological function summary
Lim Homeobox Protein 1-Like participates in processes linked to development and differentiation. It has been implicated in nervous system development possibly through interactions with transcriptional regulators. LIX1L may form part of larger protein complexes aligning with its expression in neural and reproductive tissues. Its presence in these tissues indicates roles in establishing cellular identity and tissue architecture.
Pathways
Lim Homeobox Protein 1-Like plays functions in developmental signaling pathways such as Wnt and Hedgehog. These pathways are critical for cell fate determination and tissue patterning. LIX1L might interact with proteins like beta-catenin in the Wnt signaling cascade and Gli in the Hedgehog pathway influencing their pathways and cellular outcomes.
LIX1L associates with conditions affecting the nervous system and reproductive organs. Neurological disorders like certain congenital brain malformations link with abnormal expression of LIX1L. Additionally reproductive issues possibly including infertility might arise from LIX1L's dysregulation. Studies suggest these associations through LIX1L's interactions with proteins such as GLI3 in the context of Hedgehog pathway dysregulation.