Abcam, ab258944, Human LMAN2 (VIP36) knockout HEK-293T cell lysate

Size: 1Kit
LMAN2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LMAN2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VIP36 also known as vesicular integral-membrane protein 36 operates as a lectin involved in glycoprotein transport within the cell. It has a mass of approximately 36 kDa. Scientists observe its expression mainly in the liver and testis where it localizes to the Golgi apparatus and endoplasmic reticulum. VIP36 functions to select specific N-linked glycans facilitating their sorting and transport essential for proper protein localization and function.
Biological function summary
Vesicular integral-membrane protein 36 contributes to the quality control of glycoproteins within the secretory pathway. While not forming part of a larger complex VIP36 plays a critical role in recognizing high-mannose-type glycoproteins which are critical for cell-cell communication and adhesion. This function helps maintain cellular homeostasis and impacts various cellular activities reflecting the protein's importance in cellular processes.
Pathways
Glycoprotein transport and sorting rely on VIP36 which interacts with pathways like the secretory pathway and vesicle trafficking. The protein impacts the dynamics of protein glycosylation fundamental for the correct functioning of proteins like E-cadherin. By ensuring glycoproteins reach their proper destinations within the cell VIP36 plays an important role in maintaining cellular architecture and facilitating important signaling processes.
Disruptions in VIP36 functionality might contribute to disorders like liver cirrhosis and testicular malfunction. Problems in its glycoprotein sorting capability can affect proteins such as alpha-fetoprotein potentially altering normal cellular function and leading to disease. Researchers continue to investigate VIP36's role in these disorders aiming to understand how alterations in this protein could offer insights into therapeutic targets.