Abcam, ab258945, Human LMF2 knockout HeLa cell lysate

Size: 1Kit
LMF2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 50 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 50 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LMF2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein LMF2 also known as Lipase Maturation Factor 2 plays an important role in the maturation of certain lipases. It is a small protein with a molecular mass of approximately 22 kDa. LMF2 is expressed in various tissues but shows a higher expression level in the liver and adipose tissue. It is important for the post-translational modification of lipases which are critical enzymes for lipid metabolism.
Biological function summary
LMF2 assists in the proper folding and maturation of lipases ensuring they function correctly. It acts as part of a larger maturation complex which also includes its homolog LMF1. This complex facilitates the activation of lipases that require processing before functioning properly. Mature lipases participate in breaking down lipid molecules releasing fats and other important metabolites necessary for cellular energy and storage.
Pathways
LMF2 integrates into the lipid metabolism and energy homeostasis pathways. It notably interacts with enzymes such as lipoprotein lipase which plays a critical role in hydrolyzing triglycerides in lipoproteins into free fatty acids. LMF2's function also links it to hepatic lipase activity further embedding it in the pathways that control the distribution and management of lipids across different bodily tissues.
LMF2 could potentially relate to metabolic conditions such as hyperlipidemia or obesity due to its involvement in lipid metabolism. The dysfunction or misregulation of LMF2 might disrupt normal lipid metabolism leading to abnormal accumulation of fats in the body. Moreover through its relationship with lipases such as lipoprotein lipase disruptions in LMF2's function might contribute to cardiovascular disorders given the importance of lipid processing in maintaining cardiovascular health.