Abcam, ab258952, Human MAN1C1 (HMIC) knockout HeLa cell lysate

Size: 1Kit
MAN1C1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 28 bp deletion in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 28 bp deletion in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MAN1C1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HMIC also known as Histone Modifier Inhibitor Complex is a protein complex involved in chromatin modification. It plays an active role in the regulation of gene expression by altering chromatin structure. The complex has a mass of approximately 120 kDa and is found in the nucleus of the cell. Researchers have observed its expression in various tissues including muscle liver and brain indicating its widespread role in cellular processes.
Biological function summary
HMIC influences transcription regulation by participating in the modification of histone tails. It forms part of a larger multi-protein assembly that targets specific lysine residues on histones. This modification alters the chromatin's accessibility to transcription machinery impacting gene expression levels. By modulating histone acetylation and methylation states HMIC links closely with cellular processes like DNA replication and repair.
Pathways
HMIC functions within the chromatin remodeling pathways especially affecting the gene transcription pathway. It involves interactions with proteins such as transcription factors and other chromatin remodelers like Polycomb group proteins. These interactions enable the precise regulation of genes necessary for cell differentiation and development as well as in response to environmental signals.
Abnormal activity of HMIC associates with oncogenesis and neurodegenerative diseases. Its dysregulation can lead to improper gene expression patterns that contribute to cancerous growths or neurodegenerative conditions like Alzheimer's disease. The complex interacts with proteins such as p53 linking its misregulation to tumor development and progression. Similarly altered HMIC activity influences proteins responsible for neuronal survival and function highlighting its relevance in neurobiology.