Abcam, ab258962, Human MFSD1 knockout HeLa cell lysate

Size: 1Kit
MFSD1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MFSD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MFSD1 also known as Major Facilitator Superfamily Domain Containing 1 functions as a transmembrane protein that facilitates transport across cell membranes. It displays a mass of approximately 55 kDa. MFSD1 is expressed in various tissues with notable presence in the brain liver and kidney. It localizes primarily at the lysosome suggesting its role in intracellular transport and homeostasis.
Biological function summary
MFSD1 acts as a transporter within the lysosomal membrane. It might be a part of larger lysosomal membrane complexes involved in nutrient sensing and regulation. MFSD1 contributes to maintaining cellular homeostasis by transporting small molecules across the lysosomal membrane. The precise substrates of MFSD1 remain under investigation but they likely include metabolites that are important for cellular function.
Pathways
MFSD1 operates within lysosomal transport pathways and metabolic regulation. It is associated with nutrient-sensing pathways that are essential for energy homeostasis. MFSD1 interacts with other metabolic regulatory proteins such as mTOR that play a role in cellular growth and metabolic processes. These interactions link MFSD1 to important pathways that regulate cellular metabolism and response to nutrient availability.
MFSD1 has implications in neurodegenerative conditions and metabolic disorders. Alterations in MFSD1 function may contribute to lysosomal storage disorders as it affects lysosomal transport efficiency. Additionally its interaction with mTOR relates MFSD1 to disorders like neurodegeneration where disrupted cellular homeostasis plays a significant role. Studying MFSD1 can enhance understanding of its role in diseases and offer potential therapeutic targets.