Abcam, ab258963, Human MKRN1 knockout HEK-293T cell lysate

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MKRN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 30 bp deletion in exon 1 and 3 bp deletion in exon 1 and 5 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 30 bp deletion in exon 1 and 3 bp deletion in exon 1 and 5 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MKRN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MKRN1 also known as Makorin-1 is a protein that functions as an E3 ubiquitin ligase. This protein has a molecular mass of approximately 58-60 kDa. It is highly expressed in human tissues including the brain heart and skeletal muscle. MKRN1 contains distinct zinc finger motifs that allow it to tether to its substrates aiding in target protein modification and degradation.
Biological function summary
MKRN1 regulates protein stability and turnover within cells. It plays a significant role in controlling gene expression through ubiquitination processes. MKRN1 does not form part of larger protein complexes but interacts individually with its targets. Researchers study its impact on the transcriptional activities of specific proteins given its ability to tag substrates for proteasomal degradation.
Pathways
MKRN1 participates in key cellular pathways such as cell cycle regulation and apoptosis. It interacts with proteins like p53 and ribosomal protein L6 influencing these pathways. Its ubiquitin ligase function connects it to various cellular control mechanisms essential for maintaining homeostasis and responding to cellular stressors.
Researchers established a connection between MKRN1 and cancer specifically prostate cancer. Its regulation of p53 activity influences tumor growth and cell death. Additionally studies show links between MKRN1 and neurodegenerative disorders implicating its role in protein aggregation and neuron survival. MKRN1's interaction with key proteins such as p53 and neuronal factors highlights its potential as a therapeutic target in these diseases.