Abcam, ab258973, Human MSL1 knockout HeLa cell lysate

Size: 1Kit
MSL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 1 bp insertion in exon 3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 1 bp insertion in exon 3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MSL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MSL1 or Male-Specific Lethal 1 functions mechanically as an important component of the MSL complex that engages in gene regulation activities. It has a molecular mass of approximately 62 kDa. MSL1 expresses in diverse tissues including the brain liver and muscle. It plays a critical role in dosage compensation contributing to equalizing gene expression levels between sexes in species like Drosophila.
Biological function summary
MSL1 participates in the assembly of the MSL complex. This multi-protein complex facilitates histone H4 lysine 16 acetylation which activates transcription. MSL1 specifically assists in recruiting the acetyltransferase MOF to target sites on the X chromosome. The expression level and activity of MSL1 are tightly regulated to ensure proper chromosomal balance in the organism. Its activity is not limited to dosage compensation but also influences other chromatin remodeling processes.
Pathways
MSL1 plays significant roles in chromatin modification pathways participating in transcriptional regulation actively. The protein aligns closely with MOF in these pathways forming a duo that heavily influences acetylation across chromatin landscapes. In addition to histone modification MSL1 connections extend to gene expression pathways responding to various cellular stimuli interfacing with proteins like MSL2 and MSL3 to regulate gene activation machinery.
MSL1 has associations with certain cancers including breast cancer and leukemia. The protein's dysregulation can skew chromatin remodeling leading to abnormal gene expression profiles commonly seen in tumorigenesis. MSL1 partners with other proteins like MOF impacting the transcriptional regulatory networks often targeted in cancer therapies. Understanding MSL1's role allows for targeted approaches in developing therapeutic strategies for these diseases.