Abcam, ab258989, Human NDUFA2 knockout HeLa cell lysate

Size: 1Kit
NDUFA2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDUFA2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NDUFA2 protein also known as NADH:Ubiquinone Oxidoreductase Subunit A2 is a component of the mitochondrial respiratory chain complex I. It has a mass of approximately 10.3 kDa. NDUFA2 is expressed in various tissues with high energy demands especially in the heart brain and skeletal muscles. Functionally it participates in the initial phase of electron transport by transferring electrons from NADH to ubiquinone contributing to the proton gradient used in ATP synthesis.
Biological function summary
NDUFA2 is a component of the multi-subunit complex I of the mitochondrial electron transport chain. This complex plays a role in cellular respiration by facilitating the conversion of energy stored in nutrients into ATP. Within the structure of complex I NDUFA2 collaborates with other subunits to ensure efficient electron transfer processes. Although a smaller subunit its role in the proper assembly and maintenance of the complex is essential for sustaining cellular energy levels.
Pathways
The actions of NDUFA2 are critical in oxidative phosphorylation one of the main pathways for ATP production in eukaryotic cells. This energy production pathway is intertwined with cellular respiration where NDUFA2 is related to proteins such as ND1 and ND2. These proteins collectively engage in the transfer of electrons across the inner mitochondrial membrane driving the synthesis of ATP from ADP through chemiosmotic coupling.
Alterations in NDUFA2 have connections to mitochondrial encephalomyopathy and Leigh Syndrome. These conditions manifest due to dysfunctional energy metabolism caused by defects in NDUFA2 and other proteins like NDUFS1. Mutations in these proteins can lead to impaired oxidative phosphorylation resulting in energy deficiencies that contribute to the development of neurodegenerative and muscular impairments seen in these disorders.