Abcam, ab258992, Human NDUFB1 knockout HEK-293T cell lysate

Size: 1Kit
NDUFB1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDUFB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFB1 also known as NADH:ubiquinone oxidoreductase subunit B1 is a component of complex I in the mitochondrial respiratory chain. It plays a role in the transfer of electrons from NADH to ubiquinone. NDUFB1 has a molecular mass of approximately 12.7 kDa. Expression of NDUFB1 is observed in high energy-demanding tissues like the heart brain and skeletal muscles.
Biological function summary
NDUFB1 functions as part of the mitochondrial complex I which is the first enzyme of the electron transport chain. Complex I is essential for energy production in cells as it facilitates the transfer of electrons and contributes to proton gradient formation across the inner mitochondrial membrane. NDUFB1 interacts with other subunits within complex I to ensure efficient electron flow impacting the overall respiratory activity and ATP synthesis.
Pathways
NDUFB1 is involved with the oxidative phosphorylation pathway where it collaborates with other components of complex I like NDUFS7 and NDUFV1. It also associates with the mitochondrial biogenesis pathway important for maintaining mitochondrial function and energy metabolism. Through these pathways NDUFB1 helps to sustain cellular energy balance and integrate mitochondrial signaling processes.
Defects in NDUFB1 have been connected to conditions such as mitochondrial complex I deficiency and Leigh syndrome. These diseases result from impaired energy metabolism leading to severe neurological and muscular symptoms. NDUFB1 dysfunction can influence the activity of proteins like NDUFA1 and NDUFS1 amplifying mitochondrial dysfunction and contributing to the progression of these disorders.