Abcam, ab258993, Human NDUFC1 knockout HEK-293T cell lysate

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NDUFC1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 5 bp deletion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 5 bp deletion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDUFC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFC1 also called NADH:ubiquinone oxidoreductase subunit C1 contributes to the mitochondrial respiratory chain specifically as a subunit of complex I. The protein weighs around 11 kDa and exists in the inner mitochondrial membrane. NDUFC1 participates in transferring electrons from NADH to ubiquinone playing an essential role in cellular energy production. It shows expression across various tissues supporting its wide functional significance.
Biological function summary
The electron transport chain relies on NDUFC1 for its operation as it is part of the large complex I assembly. NDUFC1 contributes by aiding the proton pump activity of complex I which is part of oxidative phosphorylation. Complex I includes about 45 subunits with NDUFC1 playing a supportive function in stabilizing this intricate structure. This efficient electron transfer and proton gradient generation drive ATP synthesis important for energy metabolism.
Pathways
Chemo-osmotic coupling in oxidative phosphorylation processes heavily relies on functional complex I and its components like NDUFC1. NDUFC1 facilitates the initial steps in the electron transport chain. It works alongside proteins such as NDUFA1 and NDUFB5 within the complex to ensure proper electron flow from NADH to ubiquinone. This integration into the oxidative phosphorylation pathway links it to other cellular processes including metabolism and reactive oxygen species regulation.
NDUFC1 often appears in discussions around mitochondrial disorders notably mitochondrial complex I deficiency which impacts energy production in cells. Mutations or dysfunctions in NDUFC1 can also contribute to neurodegenerative diseases like Leigh syndrome where defective oxidative phosphorylation leads to disrupted cellular functions. Within these disease contexts NDUFC1 interacts with proteins such as NDUFS1 in the context of complex I to influence disease severity and progression.