Abcam, ab258996, Human NEIL1 knockout HEK-293T cell lysate

Size: 1Kit
NEIL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NEIL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NEIL1 also known as Nei endonuclease VIII-like 1 functions as a DNA glycosylase involved in base excision repair. This protein has a molecular mass of approximately 44 kDa. NEIL1 operates on oxidatively damaged bases in DNA removing lesions from DNA to maintain genome integrity. Expression of NEIL1 occurs in various tissues with higher levels often found in the liver and other metabolically active organs.
Biological function summary
NEIL1 plays an important role in the DNA damage repair process ensuring stability and integrity of the genome. It is particularly active in removing oxidative damage to bases functioning as part of the base excision repair pathway. NEIL1 works in coordination with other proteins such as DNA polymerase and ligase to repair DNA. It does not exist alone but forms complexes with these and other DNA repair proteins to carry out its role efficiently.
Pathways
NEIL1 acts within the base excision repair and nucleotide excision repair pathways. These pathways are vital for correcting DNA damage caused by oxidative stress and other environmental factors. In these pathways NEIL1 interacts with other repair enzymes including DNA polymerase beta to ensure proper coordination during the repair process. The interaction helps streamline the replacement and sealing of damaged DNA segments.
Alterations in NEIL1 function associate with various conditions specifically cancer and neurodegenerative diseases. Mutations or deficiencies in NEIL1 can lead to improper repair of DNA contributing to carcinogenesis. Moreover research indicates NEIL1 may interact with other proteins like p53 a known tumor suppressor linking its role in tumor development and suppression. In neurodegenerative diseases faulty DNA repair mechanisms involving NEIL1 contribute to neuronal damage and disease progression.