Abcam, ab259002, Human NRBF2 knockout HeLa cell lysate

Size: 1Kit
NRBF2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NRBF2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NRBF2 also known as nuclear receptor binding factor 2 acts as an important regulatory protein in cellular mechanisms. It has a molecular mass of approximately 30 kDa and exhibits expression in various tissues such as the brain and liver. Mechanically NRBF2 interacts directly with the Beclin 1 complex facilitating the regulation of autophagy a process critical for cellular homeostasis and survival.
Biological function summary
NRBF2 serves essential roles in controlling autophagic vesicle formation. It forms part of the autophagy-related phosphatidylinositol 3-kinase (PI3K) complex I enhancing the lipid kinase activity required for autophagosome development. This protein-protein interaction is key for initiating the autophagy cascade ensuring that cells can manage and recycle cellular components efficiently under stress conditions.
Pathways
NRBF2 engages in the autophagy pathway where it influences the action of the PI3K complex I. It also partakes in the mTOR signaling pathway where it helps in regulating cellular energy status and protein synthesis. Through these pathways NRBF2 maintains a presence alongside other regulatory proteins like ULK1 and AMPK which mutually contribute to the balance of cell growth and metabolism.
Loss or dysfunction of NRBF2 links closely to neurological diseases and cancer. For instance its impaired function has associations with neurodegenerative disorders such as Alzheimer's disease due to its role in neuronal autophagy and associated proteins like LC3. Additionally the altered activity of NRBF2 within pathways can promote oncogenesis through connections with tumor suppressor proteins making it a point of interest in cancer research.