Abcam, ab259008, Human NUDT3 (DIPP) knockout HeLa cell lysate

Size: 1Kit
NUDT3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NUDT3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DIPP also known as a Diadenosine triphosphate hydrolase (Ap3Aase) acts mechanically as a nucleoside triphosphate diphosphohydrolase. It hydrolyzes nucleoside diphosphates such as Ap3A. DIPP has a molecular mass of approximately 41 kDa. It expresses in various tissues including the brain kidney and liver suggesting its involvement in essential biochemical processes.
Biological function summary
DIPP regulates the concentration of diadenosine polyphosphates which act as signaling molecules in cellular communication. These molecules control intracellular and extracellular nucleotide levels. DIPP sometimes forms part of a protein complex that modulates signal transduction pathways emphasizing its role in cellular homeostasis and signaling.
Pathways
DIPP integrates into the purinergic signaling pathway and it contributes to the regulation of energy metabolism. This protein interacts with other hydrolases and kinases influencing ATP degradation and synthesis. Through this interaction it plays a role with proteins like NTPDases highlighting its significance in nucleotide management.
DIPP contributes to diseases related to abnormal nucleotide levels such as cancer and neurological disorders. In cancer altered DIPP activity relates to dysregulation of cellular energy balance. Additionally in neurological disorders DIPP's interaction with proteins like Ap3A-binding proteins might affect synaptic transmission and neuronal function illustrating its involvement in neuronal health.