Abcam, ab259072, Human PXK knockout HEK-293T cell lysate

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PXK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PXK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PXK also known as Phosphoinositide-binding protein FAM20-kinase or MOYBG Protein Kinase-like is a membrane-associated protein with a molecular mass of approximately 59 kDa. Experts have identified it for its kinase-like properties although it lacks conventional kinase activity. PXK is expressed in various tissues including brain liver and kidney with significant presence in neuronal cells. Its mechanical role involves modulating neuronal signaling and membrane dynamics through interactions with phosphoinositides.
Biological function summary
PXK participates in the regulation of cellular organization and synaptic functions. While not a direct component of signaling complexes it influences processes related to cytoskeletal rearrangement and membrane trafficking. Through its phosphoinositide-binding domains PXK impacts the localization and assembly of proteins involved in synaptic vesicle turnover. As a scaffolding protein it indirectly affects signal transduction pathways by altering the spatial arrangement of interacting proteins.
Pathways
PXK influences the phosphoinositide signaling pathway and the synaptic vesicle cycle. Its interactions with proteins like dynamin and synaptojanin allow researchers to deduce its role in vesicle endocytosis and membrane recycling. PXK indirectly modulates the activity of these proteins impacting the efficiency of synaptic transmission. These pathways underline its role in maintaining synaptic stability and plasticity essential for proper neuronal communication.
Researchers have linked PXK to neurodegenerative diseases and certain immune disorders. Alterations in PXK expression have association with conditions such as Alzheimer’s disease where disrupted synaptic function is an important feature. Furthermore its interaction with immune-related proteins like NF-κB suggests a role in autoimmune processes. These links highlight the importance of PXK in maintaining cellular function and its potential as a therapeutic target in related diseases.