Abcam, ab259074, Human RAB1B knockout HEK-293T cell lysate

Size: 1Kit
RAB1B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RAB1B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB1B also known as Ras-related protein Rab-1B is a member of the Rab family of small GTPases. This protein weighs approximately 22.6 kDa and is expressed in the cytoplasm. Mechanically RAB1B regulates vesicular trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. This involves ensuring the proper budding movement and fusion of transport vesicles facilitating efficient transportation of proteins and lipids necessary for cellular function.
Biological function summary
RAB1B plays a role in maintaining cellular homeostasis and protein secretion. It is an important component of the protein transport machinery participating in vesicle coat complex formation. It regulates the movement between the ER and Golgi working closely with other Rab proteins and their effectors to keep the flow of materials steady. The localization and activity of RAB1B influence processes important for cell growth and differentiation.
Pathways
RAB1B operates significantly in the secretory and trafficking pathways. It connects with the Golgi apparatus and ER pathways participating in sorting and cellular signaling. RAB1B interacts with other proteins such as COPII and Golgi SNARE proteins to facilitate transport vesicle movement. These proteins and pathways ensure precise and organized intracellular cargo traffic impacting multiple cellular processes directly tied to the secretory pathway.
Disruptions in RAB1B function can connect to neurodegenerative diseases and cancers. Altered expression can affect protein transport linked to Alzheimer's disease where its dysregulation impacts ER-Golgi pathway proteins like APP. In various cancers abnormal RAB1B levels can influence proliferative signals and cellular stress responses. Understanding RAB1B's role helps to delineate its involvement in these diseases offering targets for therapeutic development.