Abcam, ab259082, Human REPIN1 (AP4) knockout HEK-293T cell lysate

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REPIN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 1 and 2 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 1 and 2 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-REPIN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AP4 also known as Adaptor Protein Complex 4 plays an important role in the transport of molecules within the cell. It operates as part of the family of adaptor protein complexes involved in trafficking cargo proteins between membranes. AP4 is a medium-sized protein complex with a mass ranging approximately from 300 to 500 kDa. It predominantly expresses itself in neuronal tissues where it facilitates intracellular trafficking and protein sorting essential for cellular functions.
Biological function summary
AP4 is an essential piece of the vesicular transport machinery. It interacts with various cargo proteins sorting them into distinct vesicles for different cellular destinations. AP4 functions as part of a complex that includes other adaptins and accessory proteins each contributing specialized roles for accurate protein sorting. These interactions play significant roles in maintaining cellular homeostasis and normal neuronal function.
Pathways
AP4 is intimately involved in the endosomal-lysosomal pathway and clathrin-mediated endocytosis. These pathways are important for proper cellular protein recycling and degradation processes. AP4 also associates with other proteins like AP1 and AP2 which overlap in the sorting at different cellular locations. These connections make AP4 an essential component of the broader protein transport network within cells.
Research links AP4 to certain neurodegenerative conditions and hereditary spastic paraplegia. Mutations in AP4-related genes can disrupt normal protein trafficking affecting neuronal health potentially through a combined action with the SNX3 protein. AP4’s dysfunction in these contexts illustrates its importance in maintaining neural connectivity and function highlighting its potential as a therapeutic target in related disorders.