Abcam, ab259084, Human RFWD3 knockout HeLa cell lysate

Size: 1Kit
RFWD3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RFWD3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RFWD3 also known as RNF201 is an E3 ubiquitin-protein ligase with a mass of approximately 75 kDa. This protein adds ubiquitin to specific substrate proteins marking them for degradation by the proteasome. RFWD3 is mainly expressed in the cell nucleus where it plays a role in maintaining genomic stability. The ubiquitination process mediated by RFWD3 is critical for its function in DNA repair mechanisms especially in response to DNA damage.
Biological function summary
RFWD3 is key in the context of DNA repair and cell cycle regulation. It acts in conjunction with other repair proteins forming complexes necessary for homologous recombination repair. This protein interacts with replication protein A (RPA) during DNA damage response facilitating repair by driving proper assembly and disassembly of DNA repair complexes. RFWD3 ensures the preservation of genomic fidelity and cell viability under conditions of stress.
Pathways
RFWD3 plays a central role in DNA damage response and repair mechanisms. It is directly involved in the homologous recombination pathway working alongside proteins such as CHK1 and BRCA1. RFWD3 contributes to the stability of these pathways enabling error-free repair of DNA double-strand breaks. The protein's function in these pathways is critical for the prevention of chromosomal aberrations and cancerous transformations.
RFWD3 has been linked to cancer and Fanconi anemia. Defects in RFWD3 can lead to impaired DNA repair processes resulting in genomic instability and promoting carcinogenesis. Additionally it plays a role in the Fanconi anemia pathway interacting with proteins like FANCD2 and FANCI. This interaction is essential for proper DNA crosslink repair and deficiencies can contribute to the development of Fanconi anemia characterized by bone marrow failure and increased cancer risk.