Abcam, ab259086, Human RIOK3 knockout HeLa cell lysate

Size: 1Kit
RIOK3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RIOK3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RIOK3 also known as RIO kinase 3 or SUDD is a serine/threonine kinase with a molecular mass of approximately 65 kDa. This protein acts as an atypical kinase within the RIO kinase family and localizes to the cytoplasm. RIOK3 shows expression in various tissues with higher levels notably in the immune system including the spleen and thymus. Unlike typical kinases RIOK3 contains a unique winged-helix domain that contributes to its diverse functions in cellular processes.
Biological function summary
RIOK3 participates in immune response regulation and cellular signal transduction. It does not form part of any known large protein complex but interacts with several other proteins through transient associations. By modulating pathways involved in immune processes RIOK3 affects the activation state of various immune cells. It is involved in the regulation of interferon production where it works closely with other signaling proteins to modulate responses to viral infections.
Pathways
RIOK3 serves a role in the innate immune response and RNA processing pathways. It influences the retinoic acid-inducible gene I (RIG-I) pathway where it interacts with proteins such as RIG-I and MAVS to mediate antiviral responses. Additionally RIOK3 plays a modulatory role in mRNA processing connecting with proteins involved in RNA maturation and internationalization which suggests its involvement in maintaining proper RNA homeostasis under stress conditions.
RIOK3 has associations with autoimmune conditions and viral infections highlighting its role in immune regulation. Aberrant functioning of RIOK3 can relate to systemic lupus erythematosus (SLE) where it influences the overproduction of interferons exacerbating the disease phenotype. Furthermore RIOK3 modulates responses to hepatitis C virus infections interacting with proteins such as RIG-I to control viral replication and immune evasion. Understanding RIOK3's functions can lead to insights into therapeutic interventions for these conditions.