Abcam, ab259094, Human RTP4 knockout A549 cell lysate

Size: 1Kit
RTP4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon2.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon2.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RTP4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RTP4 also known as Receptor Transporter Protein 4 plays a significant role in the endoplasmic reticulum where it facilitates the trafficking and proper function of olfactory receptors. It has a molecular mass of approximately 31 kDa. RTP4 expression is mainly found in the olfactory sensory neurons but also in various other tissues such as the brain and liver. It helps in guiding G protein-coupled receptors (GPCRs) to the cell membrane ensuring their functional expression.
Biological function summary
The RTP4 protein aids in the transport and functional expression of GPCRs without forming part of any known stable complexes. This chaperone activity is vital for GPCR function impacting signal transduction by promoting proper receptor folding and surface expression. RTP4 ensures that olfactory and other related receptors can adequately respond to stimuli. Its chaperone activity is important for maintaining GPCR dynamics in various cell types.
Pathways
RTP4 significantly influences GPCR signaling pathways and olfactory signaling pathways. It directly affects the transport and activation of olfactory receptors key components in the olfactory signaling pathway. RTP4's interaction with olfactory receptors impacts the downstream components such as adenylyl cyclase leading to successful signal transduction. Additionally RTP4 interacts with proteins in GPCR signaling that modify receptor availability on the cell surface.
RTP4 has implications in anosmia the loss or impairment of the sense of smell and potentially in liver-related disorders. Disruption of RTP4 function can lead to defective olfactory receptor trafficking resulting in anosmia. RTP4-related pathways implicate proteins like OR51E1 in olfactory dysfunctions. There may be a less clear association with liver disorders involving misfolded GPCRs; however this link requires further exploration.