Abcam, ab259100, Human SCYL2 knockout HeLa cell lysate

Size: 1Kit
SCYL2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 5 bp deletion in exon 3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 5 bp deletion in exon 3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCYL2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCYL2 also known by the name SCY1-like 2 is a member of the SCY1-like family of proteins. It has a mass of approximately 110 kDa. SCYL2 is an accessory protein with kinase-like activity but lacks enzymatic function. The protein localizes mainly in the trans-Golgi network and the cytoplasm. It is expressed in various tissues including brain and heart indicating its potential roles in different organs and systems within the body.
Biological function summary
The human SCYL2 protein is involved in cellular trafficking and is part of the coatomer complex which assists in vesicle formation and transport. It helps in clathrin-mediated endocytosis which is important for internalizing molecules like lipids and proteins. SCYL2 interacts with other proteins such as clathrin heavy chain and auxilin demonstrating its role in maintaining cellular homoeostasis and membrane dynamics.
Pathways
SCYL2 participates in the endocytosis and intracellular transport processes. It plays a part in the AP-2 complex pathway which is essential for the vesicle docking and cargo selection. Additionally SCYL2 associates with huntingtin an important protein involved in cellular interactions vital for neuron function and integrity connecting its role to neurological processes.
SCYL2 shows associations with neurological conditions like Huntington's disease. Disruptions in its regulatory functions can affect protein transport and neuron stability potentially leading to neurodegenerative symptoms. SCYL2 also interacts with tau proteins which are relevant in Alzheimer's disease indicating its potential influence in tauopathy-related neuronal deterioration.