Abcam, ab259104, Human SECTM1 knockout A549 cell lysate

Size: 1Kit
SECTM1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon2 and 20 bp deletion in exon2.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon2 and 20 bp deletion in exon2.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SECTM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SECTM1 also known as Secreted and Transmembrane 1 and sometimes as K12 is a protein with a molecular mass of approximately 28 kDa. It expresses both membrane-bound and soluble forms found mainly in epithelial cells and in some types of immune cells such as monocytes. SECTM1 resides in the Golgi apparatus and can be secreted outside the cell indicating its role in cell signaling and communication.
Biological function summary
The protein plays a role in modulating immune responses. SECTM1 interacts with CD7 a cell surface glycoprotein found on T cells and natural killer cells influencing their activation and proliferation. This interaction is important for immune system regulation and SECTM1 can function together with other immune-related proteins but usually operates independently rather than as part of a larger protein complex.
Pathways
SECTM1 is involved in immunological pathways particularly those governing T-cell activation and the broader inflammatory response. Within these pathways SECTM1 connects with proteins like CD7 and is associated with the PI3K/Akt signaling cascade. This linkage influences cell survival and growth bridging innate and adaptive immunity.
SECTM1 has associations with several inflammatory and autoimmune conditions as well as cancer. Abnormal expression levels of SECTM1 have implications in disorders such as rheumatoid arthritis where its interaction with CD7 appears to play a role in disease progression. Similarly in certain cancers altered SECTM1 expression leads to changes in immune cell behavior affecting tumor growth and metastasis.