Abcam, ab259111, Human SHC3 (SHCC) knockout HeLa cell lysate

Size: 1Kit
SHC3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon4.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon4.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SHC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHC3 also known as SHCC is an adapter protein with a molecular weight approximately 55 kDa. It plays a significant role in signal transduction pathways by mediating interactions between activated tyrosine kinases and downstream molecules. SHC3 contains typical SH2 and PTB domains which allow it to bind phosphotyrosine residues on activated receptors. This protein is mainly expressed in the neural tissues suggesting a role in nervous system functions.
Biological function summary
SHC3 acts as a mediator in the signaling pathways that regulate cell proliferation differentiation and survival. It forms part of multi-protein complexes involved in transducing signals from the cell surface to the nucleus. Upon activation SHC3 interacts with various signaling proteins to propagate essential cellular signals that contribute to neuronal development and function. These interactions highlight its importance in maintaining cellular communication within neural environments.
Pathways
SHC3 is intricately involved in the MAPK/ERK pathway and PI3K/AKT pathway both critical for cell survival and growth. In the MAPK/ERK pathway SHC3 links receptors like Trk to downstream effectors facilitating signal transmission. It also interacts with important proteins such as Grb2 and Ras serving as a scaffold to support signaling cascades that regulate neural cell function. Through these pathways SHC3 plays an integral part in linking external cellular cues to fine-tune intracellular responses.
SHC3 has connections to neurodegenerative disorders and cancer. Abnormal activity or expression of SHC3 may contribute to neurodegenerative diseases by disrupting normal signal transduction in neurons. Additionally in certain cancers altered SHC3 signaling can influence tumor growth and survival by interacting with proteins such as PI3K. Understanding SHC3's role in these conditions could advance therapeutic strategies targeting aberrant signaling pathways.