Abcam, ab259146, Human SNRNP40 knockout HEK-293T cell lysate

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SNRNP40 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 8 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 8 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SNRNP40, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SNRNP40 also known as U5-40K or Splicing Factor 40 kDa is a component of the U5 small nuclear ribonucleoprotein complex. It has a mass of approximately 40 kDa. SNRNP40 is mainly expressed in the nucleoplasm of eukaryotic cells where it plays a critical role in RNA processing.
Biological function summary
SNRNP40 participates in the pre-mRNA splicing process as part of the spliceosome a large and dynamic ribonucleoprotein complex. The spliceosome facilitates the removal of introns from pre-mRNA transcripts. SNRNP40 as a part of this complex influences the conformational changes necessary for the accurate assembly and function of the spliceosome. Through this action SNRNP40 helps maintain genomic stability and proper gene expression.
Pathways
SNRNP40's role in the spliceosome places it within essential RNA processing pathways including the mRNA splicing pathway. It interacts with other proteins such as PRPF8 and EFTUD2 (also known as Snu114) which are key components of the U5 particle. These interactions illustrate how SNRNP40's activity is integral to the functioning of the spliceosomal machinery.
SNRNP40 has been connected to certain genetic conditions through its role in RNA splicing. Defects in the splicing process can result in splicing-related disorders such as retinitis pigmentosa although SNRNP40 itself is not directly implicated in this disease. However it shares functional associations with proteins like PRPF31 which is linked to such disorders. Additionally SNRNP40 may play a role in cancer development due to its involvement in mis-splicing events affecting tumor suppressor genes and oncogenes.