Abcam, ab259173, Human TAF12 knockout HeLa cell lysate

Size: 1Kit
TAF12 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TAF12, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAF12 also known as Transcription initiation factor TFIID subunit 12 is a critical component of the transcription machinery specifically within the TFIID complex. This protein weighs approximately 22 kilodalton and acts as a bridge connecting different proteins within the transcriptional complex. TAF12 shows expression in various tissues with higher levels observed in actively dividing cells like those in the immune system or during embryonic development. Its expression pattern suggests a role in processes that require rapid gene expression changes.
Biological function summary
TAF12 contributes to the formation and stability of the TFIID complex which is essential for the initiation of transcription by RNA polymerase II. As part of this complex TAF12 helps recognize and bind to the core promoter elements of genes facilitating the assembly of the pre-initiation complex. This process drives gene expression that is responsive to various physiological signals. TAF12's interaction with other TAF (TBP-associated factors) proteins ensures precision in the selection of transcription start sites.
Pathways
TAF12 integrates into gene expression pathways affecting cellular proliferation and differentiation. Its role in the canonical Wnt signaling pathway is significant a pathway that regulates important aspects of development and cell signaling. TAF12 also interacts with proteins such as TAF1 and TBP which jointly influence transcription initiation. These protein interactions are fundamental for the regulation of genes that control cell cycle progression and response to external growth signals.
TAF12's function relates closely to cancer and neurodegenerative diseases. Its overexpression or misregulation may lead to oncogenesis as aberrant transcription can drive uncontrolled cell proliferation. In cancers TAF12's interaction with other transcription factors like the ETS family might contribute to tumorigenesis. Additionally alterations in TAF12 might be implicated in neurodegenerative disorders like Alzheimer's disease where the regulation of neuronal gene expression is disrupted. Studies are ongoing to understand fully how TAF12 and its associated proteins influence disease pathology and progression.