Abcam, ab259179, Human TIMM17B knockout HEK-293T cell lysate

Size: 1Kit
TIMM17B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon3 and Insertion of the selection cassette in exon3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon3 and Insertion of the selection cassette in exon3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TIMM17B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TIMM17B also known as Translocase of Inner Mitochondrial Membrane 17 homolog B is a protein component with a molecular mass of approximately 21 kDa. TIMM17B is importantly involved in the translocation of proteins across the mitochondrial inner membrane. This protein is expressed in various tissues but shows higher expression in testis and heart. TIMM17B plays a critical role in mitochondrial function by acting as a channel-forming protein within the TIMM complex.
Biological function summary
TIMM17B integrates its function into the import machinery for mitochondrial proteins. It is part of the TIM22 complex an important multi-protein system that mediates the insertion and assembly of certain hydrophobic proteins into the mitochondrial inner membrane. TIMM17B works in coordination with other TIMM proteins such as TIM22 to facilitate the transfer and integration of mitochondrial carrier proteins. This integration supports the effective maintenance of mitochondrial functions which are essential for cellular energy metabolism.
Pathways
TIMM17B plays a significant role in the mitochondrial protein import pathway. This process involves the transport of proteins synthesized in the cytosol into the mitochondria which is essential for mitochondrial biogenesis and function. TIMM17B is also linked with the oxidative phosphorylation pathway through its involvement in maintaining mitochondrial membrane potential. Proteins like TIMM23 and TIMM50 are closely related to TIMM17B within these pathways providing important support for its function in importing proteins necessary for energy production.
TIMM17B has been connected to mitochondrial disorders and neurodegenerative diseases. Disturbances in its function or expression can affect mitochondrial integrity contributing to conditions such as Leigh syndrome. Moreover TIMM17B shows associations with Alzheimer's disease where defects in mitochondrial dynamics and energy metabolism play a role. These connections also involve the protein PINK1 which is associated with autosomal recessive early-onset Parkinson's disease highlighting the importance of mitochondrial quality control in neurodegenerative disorders.