Abcam, ab259186, Human TMEM147 knockout HEK-293T cell lysate

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TMEM147 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM147, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM147 also known as NBR1 is a transmembrane protein with a molecular mass of approximately 28 kDa. It resides primarily in the endoplasmic reticulum and is widely expressed across various tissues such as the brain and liver. The protein spans the membrane multiple times indicating its role in maintaining cellular structure and function within the membrane environment. TMEM147 is often associated with regulatory processes that affect membrane-related activities.
Biological function summary
TMEM147 interacts with proteins in the ribosome maturation process and is involved in assembling the nuclear pore complex. It forms part of the N-oligosaccharyltransferase (OST) complex which plays an essential role in protein modification through glycosylation. This complex activity helps in the proper folding and stability of proteins necessary for cell function. Therefore TMEM147 influences cellular processes by regulating the maturation and transport of proteins within cells.
Pathways
TMEM147 contributes significantly to the N-glycosylation and protein biosynthesis pathways. Within these pathways the protein interacts with enzymes like STT3A and STT3B which are key catalytic components of the oligosaccharyltransferase complex. Through this interaction TMEM147 ensures proper protein folding and efficiency in glycoprotein processing. It also influences cellular homeostasis and stress responses by coordinating with proteins related to ER stress pathways.
TMEM147 shows a connection to neurodegenerative diseases and certain types of cancer. Studies highlight its involvement in Alzheimer's disease where it interacts with the γ-secretase complex including proteins like presenilin-1 (PSEN1) affecting amyloid-beta precursor protein processing. In cancer abnormal TMEM147 expression correlates with altered N-glycosylation patterns leading to changes in tumor cell behavior. This association suggests TMEM147's potential role as a biomarker or therapeutic target in disease management.