Abcam, ab259192, Human TMEM64 (TMM64) knockout A549 cell lysate

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TMEM64 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 20 bp deletion in exon1 and 5 bp deletion in exon1.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 20 bp deletion in exon1 and 5 bp deletion in exon1.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM64, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMM64 also known as Transmembrane Protein 64 is a protein that interacts with various cellular mechanisms. It has a molecular weight of approximately 64 kDa. This protein localizes within both the cytoplasmic and membrane regions in several cell types. Most notably it shows higher expression in adipose tissues and certain layers of the skin indicating its role in specific physiological processes related to these tissues.
Biological function summary
TMM64 interacts with molecular complexes that regulate lipid metabolism and energy homeostasis. Within cells this protein contributes to the regulation of cellular energy status by modulating interactions between lipid droplets and mitochondria. It influences lipid storage and usage by affecting enzymes involved in lipid biosynthesis pathways. This suggests its involvement in energy balance and metabolic processes.
Pathways
TMM64 has significant roles in the lipid metabolism and insulin signaling pathways. Within these pathways it acts in conjunction with proteins such as AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptors (PPARs). In the lipid metabolism pathway TMM64 modulates the response to metabolic signals assisting in the adaptation of lipid storage and mobilization. It influences how cells react to insulin signals affecting glucose uptake and storage as lipids.
TMM64 appears significant in conditions like obesity and type 2 diabetes mellitus. Dysregulation of TMM64 expression or function correlates with increased lipid accumulation and impaired insulin action. This association relates TMM64 to insulin receptor substrate 1 (IRS1) and its role in insulin sensitivity. By influencing pathways critical to lipid and glucose homeostasis alterations in TMM64 may contribute to the development and progression of metabolic diseases.