Abcam, ab259196, Human TNKS1BP1 (Tankyrase binding protein 1) knockout HeLa cell lysate

Size: 1Kit
TNKS1BP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TNKS1BP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tankyrase binding protein 1 also known as TAB182 or TNKS1BP1 interacts directly with tankyrase enzymes. It has a molecular mass of approximately 182 kDa. The protein mainly localizes in the cytoplasm and nucleus. Its interaction with tankyrase a poly(ADP-ribose) polymerase modulates various cell processes. This binding suggests TAB182 has important roles in managing protein stability and cellular localization reactions.
Biological function summary
TAB182 participates heavily in cellular regulation activities. It forms complexes with tankyrase contributing to the control of telomere length and maintenance. By engaging with numerous other proteins TAB182 influences functions like Wnt signaling and Axin degradation. It interacts with tankyrase to impact cellular structures like centrosomes hinting at a broad regulatory role in cell cycle regulation.
Pathways
TAB182's interaction with tankyrase places it within notable cellular pathways. It plays a role in the Wnt/β-catenin signaling pathway which regulates gene transcription and cellular differentiation. In this pathway TAB182 works closely with Axin and the destruction complex. Furthermore TAB182 is linked to the telomere elongation pathway collaborating with TRF1 to regulate telomere maintenance inheriting tankyrase association roles.
TAB182 connection implicates it in cancer and metabolic disorders. In cancer TAB182 dysregulation might alter telomere maintenance facilitating oncogenesis in conjunction with proteins like TRF1. Regarding metabolic disorders disruptions in Wnt signaling pathways involving TAB182 and Axin could contribute to obesity or type 2 diabetes as these pathways regulate adipogenesis and insulin sensitivity. Understanding TAB182's role helps uncover potential therapeutic targets for these diseases.