Abcam, ab259247, Human ZFR knockout HEK-293T cell lysate

Size: 1Kit
ZFR KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 131 bp deletion in exon4.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 131 bp deletion in exon4.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZFR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The zinc finger RNA-binding protein commonly known as ZFR functions mechanically as an RNA-binding protein that plays a role in RNA processing and regulation. ZFR with a molecular mass of around 115 kDa contains zinc finger motifs that facilitate its binding to RNA. It is widely expressed across various tissues with significant expression in brain and muscle tissues. ZFR interacts specifically with RNA molecules to influence their stability and processing.
Biological function summary
ZFR regulates several processes related to RNA metabolism including splicing and degradation important for maintaining cellular RNA homeostasis. ZFR also participates as a subunit in larger ribonucleoprotein complexes which are essential for post-transcriptional gene regulation. Its activity in facilitating RNA transport and stability links it to essential cellular processes impacting gene expression at the post-transcriptional level.
Pathways
ZFR engages in regulatory networks that include pathways involved in RNA processing and stabilization. It plays a part in mRNA splicing pathways important for producing mature mRNA molecules from pre-mRNA. This interaction closely relates ZFR to other proteins such as SMN and other splicing factors integrating ZFR into broader gene expression and RNA management networks.
Alterations in ZFR expression or function have links to neurological conditions such as amyotrophic lateral sclerosis (ALS). This link is through the dysregulation of RNA-binding proteins like TDP-43 which ZFR connects with in RNA metabolic pathways affected in ALS. Additionally ZFR-related anomalies also show associations with certain muscular disorders due to its high expression in muscle and its role in RNA processing within these tissues.