Abcam, ab259249, Human ZNF326 knockout HeLa cell lysate

Size: 1Kit
ZNF326 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZNF326, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZNF326 also known as Znf20 or Zinc Finger Protein 326 functions as a transcriptional regulator influencing gene expression by binding to DNA. It is part of the Krüppel-associated box (KRAB) zinc finger protein family and has a molecular mass of approximately 49 kDa. ZNF326 is expressed in multiple tissues including brain heart and pancreas indicating its broad role in cellular activities.
Biological function summary
This protein modulates RNA processing and transcriptional regulation often working as part of a transcriptional complex. It interacts with other molecules to facilitate the splicing and processing of pre-mRNA impacting the stability and translation of messenger RNA. This role in RNA biology highlights its importance in maintaining proper cellular function and gene expression profiles.
Pathways
Studies show that ZNF326 participates in the RNA splicing and transcription regulation pathways affecting the expression and function of various genes. It is notably connected with the spliceosome pathway which is important for accurate RNA processing. ZNF326’s function alongside proteins like the ATP-dependent RNA helicase DDX5 and serine/arginine-rich splicing factor 1 (SRSF1) ensures proper assembly and function of spliceosomal components.
Research has linked ZNF326 to neurodegenerative disorders and certain cancers such as glioblastoma. Abnormal activity or expression of ZNF326 disrupts mRNA metabolism contributing to the pathogenesis of these diseases. In glioblastoma ZNF326 works alongside proteins like p53 to influence cellular proliferation and apoptosis thereby playing a role in tumor development and progression.