Abcam, ab259381, Recombinant human IL-6 protein (Active)

Size: 10µg / 50µg / 100µg / 250µg
Recombinant human IL-6 protein (Active) is a Human Full Length protein, in the 28 to 212 aa range with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, Functinonal studies, ELISA, Cell Culture and more. The predicted molecular weight of ab259381 protein is 21 kDa. - Save time and ensure accurate results - use our IL-6 protein as a control - Optimal protein bioactivity, stability and reproducibility - Available in different sizes to fit your experimental needs
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 0.005 EU/µg,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGE, Cell Culture, sELISA, HPLC, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Proliferation of TF-1 cells is 0.8067 ng/mL corresponding to a specific activity of 1.24 x 106 IU/mg.,
Accession:P05231,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in PBS,
Storage buffer:pH: 6 - 8Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Product details:
Ensure the validity of your result using our recombinant human IL-6 protein ab259381 as a control.
The ab259381 IL-6 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC. Analyze your IL-6 ELISA data using the ab259381 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE
Check out our ELISA protocol for more information
Premium Bioactive Protein range
The ab259381 IL-6 protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
More premium bioactive proteins can be found

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.
Biological function summary
IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.
Pathways
IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.
IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.