Abcam, ab259602, Anti-H6PD/GDH antibody [EPR22043-34] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal H6PD/GDH antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22043-34,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab259602 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse GDH. The recommended pair for sandwich ELISA is:
Capture:
ab259601
, Mouse GDH Capture Antibody (unconjugated)
Detector: ab259602, Mouse GDH Detector Antibody (unconjugated)
The reference range value is 0.3125 - 30 ng/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
H6PD also known as glucose dehydrogenase (GDH) or G.D.H. is an enzyme with an important mechanical role in cellular metabolism. It has a molecular mass of approximately 92 kDa. This enzyme participates in the reversible oxidation of glucose and other hexose sugars an action that results in the generation of NADPH. H6PD is expressed mainly in the endoplasmic reticulum of various tissues including liver adrenal gland and adipose tissue where it facilitates the pentose phosphate pathway and other metabolic functions.
Biological function summary
H6PD influences multiple metabolic processes by maintaining cellular redox balance through NADPH production. It plays a role in carbohydrate metabolism and aids in lipid biosynthesis and detoxification processes. Although H6PD does not form a complex it works closely with 11β-hydroxysteroid dehydrogenase type 1 an enzyme involved in corticosteroid metabolism. This interaction supports the regulation of local cortisol concentrations via the interconversion of active and inactive forms.
Pathways
Several metabolic pathways integrate the function of H6PD including the pentose phosphate pathway and the gluconeogenesis pathway. In the pentose phosphate pathway H6PD contributes to the generation of ribose-5-phosphate a precursor for nucleotide synthesis. Related proteins like glucose-6-phosphate dehydrogenase (G6PD) also share roles in these pathways working together to ensure the proper supply of reducing agents like NADPH needed for anabolic reactions.
H6PD has associations with metabolic disorders such as cortisone reductase deficiency and type 2 diabetes. Its role in cortisone metabolism connects it closely with 11β-hydroxysteroid dehydrogenase type 1 which can lead to deregulation of corticosteroid activity and contribute to insulin resistance and obesity. Understanding the connections between H6PD function and metabolic syndrome aids in revealing the complex landscape of metabolic control and its clinical implications.