Abcam, ab259631, Anti-MCP5 antibody [EPR20975-251] - BSA and Azide free (Capture)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MCP5 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20975-251,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab259631 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MCP-5. The recommended pair for sandwich ELISA is:
Capture: ab259631, Mouse MCP-5 Capture Antibody (unconjugated)
Detector:
ab259632
, Mouse MCP-5 Detector Antibody (unconjugated)
The reference range value is 27.56 - 1700 pg/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MCP-5 also known as CCL12 is a chemokine protein that plays a role in immune response by recruiting and activating leukocytes. It has a molecular mass of approximately 12 kDa. This protein is expressed mainly in tissues related to the immune system including the spleen and liver. MCP-5 exhibits a sequence homology with other members of the C-C chemokine family and shares functional similarities with MCP-1.
Biological function summary
MCP-5 takes part in promoting the migration of monocytes and lymphocytes to sites of inflammation. This chemokine is not a part of a complex but interacts with specific receptors on target cells such as CCR2. Through these interactions MCP-5 helps mediate a range of immune responses enhancing both innate and adaptive immune activities. It also contributes to the clearance of pathogens by guiding leukocytes to infection sites.
Pathways
MCP-5 plays an important role in the chemokine signaling pathway and the inflammatory response pathway. Through chemokine signaling MCP-5 interacts with CCR2 facilitating the movement of immune cells to areas of tissue damage or infection. This regulation of cell traffic is essential for maintaining homeostasis and responding to pathological changes in tissues. Another protein connected to MCP-5 in these pathways is MCP-3 which shares overlapping functions in immune cell recruitment.
MCP-5 has been implicated in conditions such as asthma and rheumatoid arthritis. In these disorders MCP-5 contributes to the recruitment of inflammatory cells worsening symptoms. It also shows a connection with other proteins like MCP-1 which similarly aids in the movement of monocytes to inflammatory sites. The presence and activity of MCP-5 and its related proteins can therefore represent potential targets for therapeutic interventions in controlling excessive inflammatory responses.