Abcam, ab259969, Anti-KCC2 antibody [EPR24203-85]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal KCC2 antibody. Suitable for ICC/IF, IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24203-85,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, IHC-Fr, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KCC2 also known as the potassium-chloride cotransporter 2 or SLC12A5 is an important neuronal protein. This transporter weighs approximately 125 kDa and is predominantly found in the central nervous system especially in neurons. KCC2 plays a central role in maintaining the intracellular chloride ion concentration by extruding chloride ions out in exchange for potassium ions. Through this action it aids in establishing the inhibitory action of neurotransmitters like GABA and glycine.
Biological function summary
KCC2 contributes significantly to the regulation of neuronal excitability and synaptic transmission. It does not appear as part of a larger protein complex but functions as a homodimer. KCC2's activity helps determine the strength and efficacy of inhibitory synapses influencing processes such as synaptic plasticity and neural development. By managing chloride ion gradients KCC2 ensures proper inhibitory neurotransmission which is essential for balanced neural activity.
Pathways
KCC2 is important for the GABAergic neurotransmission pathway which is significant for inhibitory signaling in the nervous system. This transporter interacts closely with proteins like NKCC1 another cation-chloride cotransporter which has an opposing function in chloride ion movement. The GABAergic pathway relies on KCC2 for hyperpolarizing inhibition contributing to the regulation of neuronal networks and stabilizing membrane potential.
KCC2 malfunctions associate with epilepsy and neuropathic pain. Altered expression or dysfunction of KCC2 disrupts inhibitory control leading to abnormal hyperexcitability in neurons which is a characteristic of epilepsy. Moreover in neuropathic pain downregulation of KCC2 impairs glycinergic inhibition contributing to the pathophysiology of chronic pain states. In these conditions KCC2's interaction with proteins such as NKCC1 highlights its role in maintaining inhibitory balance and neural homeostasis.