Abcam, ab263147, Human CERK knockout HEK-293T cell lysate

Size: 1Kit
CERK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-CERK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CERK or ceramide kinase is an enzyme responsible for the conversion of ceramide into ceramide-1-phosphate (C1P). This enzyme has a molecular mass of approximately 66 kDa. Researchers identify CERK as 'beta CERK' due to isoform differences in some cases. CERK expression is observed in several human tissues with significant levels in the brain lungs and spleen. Under physiological conditions CERK localizes mostly to the Golgi apparatus and other intracellular membranes.
Biological function summary
CERK plays a vital role in maintaining the balance of lipid signaling molecules within cells. It acts as an important mediator in the conversion process that produces C1P a molecule involved in signaling pathways that regulate cell survival proliferation and inflammatory responses. While not part of a larger protein complex CERK often interacts with other signaling lipids and proteins to execute its biological functions effectively.
Pathways
CERK is an important component of the ceramide signaling pathway and the sphingolipid metabolism pathway. Within these pathways CERK works alongside sphingosine kinase and other enzymes to regulate the balance of sphingolipids which are critical for cell signaling and membrane structure. The signaling pathway involving CERK influences cellular responses by altering lipid signaling molecules affecting cellular dynamics including apoptosis and growth processes.
CERK has been linked to inflammatory diseases and cancer. Inflammatory disorders like rheumatoid arthritis exhibit altered CERK activity and expression affecting C1P signaling in the inflammatory processes. In cancer aberrant regulation of CERK can modify tumor cell survival and proliferation. This connection highlights its interaction with proteins such as sphingosine-1-phosphate in the disease mechanism indicating potential therapeutic targets for interventions.