Abcam, ab263243, Human KXD1 (C19orf50) knockout HEK-293T cell lysate

Size: 1Kit
KXD1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-KXD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C19orf50 also known as Chromosome 19 Open Reading Frame 50 is a protein that has a molecular weight of approximately 17 kDa. The protein expresses in various tissues throughout the body indicating a broad role within cellular activities. Though its exact mechanical function isn’t fully determined it may interact with other proteins due to its expression levels across different systems.
Biological function summary
Proteins associated with C19orf50 engage in cellular development and maintenance. Evidence suggests it acts as part of a protein complex influencing key processes within cells. Its function may intersect with regulatory mechanisms affecting cellular growth and differentiation.
Pathways
Cellular processes involving C19orf50 are linked with cellular signal transduction and metabolic pathways. The protein potentially interacts with key players in these pathways such as mTOR signaling. This connection aids in modulating cellular energy balance with influences observed alongside proteins like AMPK which play a role in energy homeostasis and cellular response to stress.
C19orf50's role involves pathogeneses related to metabolic dysfunction and proliferative disorders. In the context of type 2 diabetes evidence suggests a possible interaction with insulin signaling proteins contributing to impaired glucose metabolism. Additionally research implicates its regulatory influence in some cancers possibly through modulation of pathways involving tumor suppressor proteins like p53.