Abcam, ab263256, Human MRFAP1 knockout HEK-293T cell lysate

Size: 1Kit
MRFAP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MRFAP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRFAP1 (Morf4 family-associated protein 1) plays a role in chromatin remodeling and transcription regulation. It physically interacts with the tumor suppressor MORF4L1 (also known as Mortality Factor 4-like 1) and other members of the MRG15 complex. MRFAP1 which has a molecular weight of approximately 13.5 kDa is predominantly expressed in the nucleus of cells. Its expression is observed in various tissues but it's notably high in the testes and heart.
Biological function summary
The protein MRFAP1 contributes to the regulation of transcription by serving as a scaffold linking histone acetylation and methylation. This action fosters interactions within the MRN complex which includes MRG15. Through these connections MRFAP1 influences chromatin structure and as a result gene expression. Its role is significant in regulating cellular senescence and differentiation.
Pathways
MRFAP1 is actively involved in the chromatin modification pathway. This pathway coordinates with the action of other proteins such as MRG15 to alter histone acetylation levels impacting transcriptional regulation. Additionally MRFAP1 engages in the DNA damage repair pathway assisting another protein known as RNF8. These interactions anchor MRFAP1 in pivotal cellular functions relating to genetic stability.
MRFAP1 has associations with cancer and certain chromatin-related disorders. Cancer research highlights MRFAP1's contribution to tumorigenesis where changes in its function can disrupt chromatin remodeling leading to unchecked cell proliferation. Furthermore alterations in MRFAP1 can affect its interaction with MORF4L1 influencing cancer progression. The link between MRFAP1 and these proteins provides insights into potential therapeutic targets for treating these conditions.