Abcam, ab263271, Human MZT2B knockout HEK-293T cell lysate

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MZT2B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 29 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 29 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MZT2B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MZT2B protein also known as Mitotic Spindle-Organizing Protein 2B is a component of the mitotic spindle apparatus. It plays a role in the organization of microtubules during cell division. MZT2B has a molecular mass of approximately 20 kDa. It is expressed in various tissues with prominent expression in proliferative tissues such as those in the reproductive system and certain areas of the brain.
Biological function summary
The MZT2B protein contributes to the proper formation and function of the mitotic spindle. It is an integral part of the gamma-tubulin ring complex which is critical for microtubule nucleation at centrosomes. This complex ensures the stability and anchoring of microtubules facilitating accurate and equal chromosome segregation during mitosis. Proper spindle assembly is essential for optimal cell division and proliferation.
Pathways
The MZT2B protein plays a prominent role in the mitotic spindle assembly pathway and the cell cycle checkpoint pathway. It associates with proteins like gamma-tubulin and other components of the spindle assembly checkpoint machinery ensuring that all chromosomes are precisely aligned and attached to spindle fibers before proceeding to the next phase of cell division. The interplay between MZT2B and these pathways ensures successful mitosis and genomic stability.
Deregulation of MZT2B has been linked to various types of cancer including breast and ovarian cancer. Abnormalities in the function or expression of MZT2B can lead to chromosomal instability which is a hallmark of cancerous transformations. Changes in expression levels of MZT2B may also affect interactions with proteins like p53 an important tumor suppressor influencing cancer progression and treatment responses.