Abcam, ab263274, Human NCAPG2 knockout HEK-293T cell lysate

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NCAPG2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 23 bp deletion in exon 3 and 5 bp deletion in exon 3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and 23 bp deletion in exon 3 and 5 bp deletion in exon 3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NCAPG2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein NCAPG2 also known as Condensin-2 complex subunit G2 is important to the mechanical processes within cells. It plays an important role in mitosis and is part of the condensin II complex responsible for chromosome condensation and stabilization during cell division. NCAPG2 is a large protein with a mass of around 120 kDa. Expression is predominantly observed in proliferating tissues such as those in the bone marrow and thymus. This wide expression reveals its direct participation in fundamental cellular functions.
Biological function summary
The organism relies on NCAPG2 to ensure accurate chromosome segregation. As a component of the condensin II complex it collaborates with other subunits including NCAPD3 and SMC2. The protein's main task is to maintain the structural integrity of chromosomes allowing proper chromosome alignment during mitosis. This integrity is essential for genomic stability reducing the risks of aneuploidy.
Pathways
NCAPG2 is deeply involved in the regulation of the cell cycle. It takes part in both the mitotic prophase and anaphase pathways. Its function relates closely to other proteins like Cdc2 and Cyclin B which direct the progression of cell division. By integrating with structural pathways of chromosomes NCAPG2 becomes an important element in preventing mitotic errors and preserving cellular fidelity.
NCAPG2 has been linked to carcinogenesis and some developmental disorders. Aberrant expression or dysfunction of NCAPG2 is often observed in certain cancers such as breast and prostate cancer where genomic instability is a common characteristic. In cancerous conditions its interaction with proteins like p53—known for DNA damage response—becomes significant highlighting its potential role in tumorigenesis. Furthermore studies investigate its connection with developmental anomalies probing possible implications in aberrant cellular proliferation pathways.