Abcam, ab263275, Human NDC1 knockout HeLa cell lysate

Size: 1Kit
NDC1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2 and 2 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2 and 2 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDC1 also known as Nucleoporin NDC1 is an important component of the nuclear pore complex. It is a highly conserved protein weighing approximately 75 kDa. NDC1 localizes to the nuclear envelope. This protein integrates into the nuclear pore complex which facilitates the transport of molecules across the nuclear envelope. NDC1's structural role is significant for maintaining nuclear pore complex integrity during cell division in eukaryotic cells.
Biological function summary
NDC1 interacts with other nucleoporins to form a functional core within the nuclear pore complex. It plays a critical role in regulating the trafficking of macromolecules between the cytoplasm and nucleus. As part of a dynamic assembly NDC1 ensures that nuclear-cytoplasmic transport processes remain efficient. This protein's interaction with other nucleoporins like NUP53 and NUP93 suggests a scaffold function in pore architecture and assembly.
Pathways
NDC1 integrates into the nucleocytoplasmic transport pathway and is essential in cell cycle regulation. This pathway is important for maintaining cellular homeostasis as it manages the exchange of proteins and RNA. NDC1 works alongside proteins such as NUP205 and Gle1 to facilitate effective transport mechanisms. By supporting such pivotal processes NDC1 helps in ensuring proper cellular functionality and division.
NDC1's dysfunction associates with conditions such as cancer and developmental abnormalities. Abnormalities in NDC1 expression or modification can lead to disruptions in nuclear transport contributing to tumorigenesis. Such imbalances might affect its interaction with other proteins like p53 a known tumor suppressor. Understanding NDC1's role could provide insights into targeted therapies for these conditions.