Abcam, ab263276, Human NDUFA4 knockout HEK-293T cell lysate

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NDUFA4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 31 bp deletion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 31 bp deletion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDUFA4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDUFA4 also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 is a small protein with a molecular mass of around 9 kDa. It plays a role as part of the mitochondrial respiratory chain where it contributes to the electron transport process. NDUFA4 is expressed in various tissues but has higher levels of expression in tissues with elevated metabolic demand like the heart liver and muscle. Its location in the inner mitochondrial membrane allows it to participate directly in respiration activity by aiding electron transfer from NADH to ubiquinone.
Biological function summary
NDUFA4 functions as part of the larger protein complex known as Complex I or NADH:ubiquinone oxidoreductase. Complex I is a critical component of the mitochondrial electron transport chain. It supports ATP synthesis by creating a proton gradient across the inner mitochondrial membrane. NDUFA4 participates in this energy conversion process and cooperates with additional protein subunits in Complex I to maintain cellular energy metabolism. This makes it essential for maintaining efficient aerobic respiration.
Pathways
NDUFA4 has a significant role within the oxidative phosphorylation pathway a central metabolic pathway in cellular energy production. Within this pathway it associates with other proteins such as NDUFB8 and NDUFS1 in Complex I which work together for effective electron transport. NDUFA4 also intersects with the broader pathways of metabolic regulation lending support to the processes that produce ATP as a cellular energy currency.
Mutations or dysregulation of NDUFA4 have been connected to mitochondrial diseases such as mitochondrial myopathy. These conditions arise due to impaired energy production in cells. Additionally alterations in NDUFA4 levels or activity have implications in neurodegenerative disorders like Parkinson's disease. In such diseases proteins like NDUFS4 another Complex I subunit also become affected highlighting potential protein-protein interactions critical for maintaining mitochondrial health.