Abcam, ab263277, Human NDUFB5 knockout HeLa cell lysate

Size: 1Kit
NDUFB5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDUFB5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NDUFB5 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 is a part of the mitochondrial respiratory chain complex I. It has a molecular mass of approximately 22 kDa. This protein is expressed in tissues with high energy demands such as the brain and muscle. Complex I where NDUFB5 operates plays an important role in cellular respiration initiating the electron transport chain by transferring electrons from NADH to ubiquinone.
Biological function summary
The NDUFB5 protein functions as a subunit within a larger multi-protein enzyme complex called mitochondrial complex I. This complex consisting of 45 subunits is important for oxidative phosphorylation. NDUFB5 is involved in the transfer of electrons facilitating the generation of a proton gradient across the mitochondrial inner membrane. This proton gradient drives ATP production which is essential for energy supply in cells.
Pathways
NDUFB5 participates centrally in the oxidative phosphorylation pathway and is involved in the production of ATP from ADP. The protein's interaction with NADH and ubiquinone links it closely to cellular respiration processes. It collaborates directly with other complex I subunits such as NDUFA and NDUFS to ensure effective energy conversion. Changes in NDUFB5 function can disrupt these pathways affecting overall energy metabolism.
Changes in NDUFB5 activity often relate to mitochondrial disorders particularly those affecting muscle and neurological function such as Leigh syndrome. Mutations affecting its role in complex I can disrupt ATP production. NDUFB5’s dysfunction may connect with other complex I proteins like NDUFS2 leading to bioenergetic failure in cells. These disruptions often result in varied clinical effects highlighting the importance of NDUFB5 in health and disease contexts.