Abcam, ab263282, Human NOMO2 knockout HeLa cell lysate

Size: 1Kit
NOMO2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and 2 bp insertion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and 2 bp insertion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NOMO2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOMO2 also known as Nodal Modulator 2 or pM5 is an endoplasmic reticulum membrane protein with a molecular mass of approximately 130 kDa. It functions mechanically as a part of the Nomo family which includes homologous proteins such as NOMO1 and NOMO3. The NOMO2 protein plays a critical role in the suppression of Nodal signaling. This protein is ubiquitously expressed but with higher levels observed in tissues including the pancreas and testis.
Biological function summary
NOMO2 modulates essential signaling pathways during early embryonic development. It acts as part of a larger protein complex known as the Nicalin-NOMO complex which modulates Nodal signaling. This signaling is important for pattern formation and axis specification during embryogenesis. By participating in these processes NOMO2 contributes to the regulation of cellular differentiation and proliferation.
Pathways
NOMO2 operates within the TGF-beta signaling pathway an important player in cell growth and differentiation. It directly influences Nodal signaling a branch of the TGF-beta pathway by interacting with other proteins like Nicalin and TMEM147. Through its involvement in these pathways NOMO2 helps coordinate cellular responses and genetic transcription important for embryonic patterning and organogenesis.
NOMO2 is linked to congenital disorders resulting from disrupted embryonic development. Alterations in NOMO2 function can contribute to conditions such as congenital heart defects. Additionally NOMO2's connection with the TGF-beta pathway may influence cancer progression as this pathway frequently interacts with oncogenes and tumor suppressors like SMAD proteins that are dysregulated in cancer.