Abcam, ab263283, Human NRD1 (Nardilysin) knockout HEK-293T cell lysate

Size: 1Kit
NRDC KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NRDC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nardilysin also referred to as NRD1 is a metalloendopeptidase that plays a role in protein processing. It possesses a molecular weight of approximately 124 kDa. Nardilysin is part of the M16 family of peptidases and is located predominantly in the cytoplasm but can also be found in the cell nucleus. The protein exhibits expression in a wide range of tissues indicating its widespread functional importance.
Biological function summary
Nardilysin impacts several cellular processes due to its role in cleaving specific peptides. It serves as a modulator of protein-protein interactions and is involved in the regulation of cell proliferation and differentiation. Nardilysin participates in the processing of various bioactive peptides and proteins and can function as part of a protein complex in some contexts. Its regulatory activities suggest its important role in maintaining cellular function and integrity.
Pathways
Nardilysin is involved in the promotion of the fibroblast growth factor (FGF) pathway and the insulin-like growth factor (IGF) signaling pathway. In these pathways it interacts with other proteins such as FGF receptors and IGF-binding proteins influencing cellular growth signaling and metabolic regulation. Nardilysin's involvement highlights its capacity to modulate key biological processes which are essential for normal cellular responses.
Nardilysin is linked with neurological disorders such as Alzheimer's disease where it is known to interact with amyloid precursor protein (APP) which plays a significant role in the disease's pathogenesis. Moreover its dysregulation has associations with metabolic disorders like obesity impacting proteins such as leptin and affecting energy balance. These associations emphasize the clinical significance of nardilysin as a potential target for therapeutic intervention.