Abcam, ab263284, Human NRG2 knockout HeLa cell lysate

Size: 1Kit
NRG2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NRG2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NRG2 protein also known as neuregulin 2 belongs to the neuregulin family and is involved in cell communication. It possesses a mass of approximately 44 kDa. NRG2 is present in various tissues with notable expression in the central nervous system heart and skeletal muscles. Its structure includes an epidermal growth factor (EGF)-like domain which enables interaction with other proteins on cell surfaces.
Biological function summary
NRG2 is involved in neural development and synaptic plasticity. It associates with ErbB receptor tyrosine kinases to mediate cellular responses. NRG2 forms a complex with these receptors to trigger downstream signaling pathways that support nerve cell growth and repair. As a ligand it selectively binds to ErbB3 and ErbB4 initiating pathways important for brain homeostasis and function.
Pathways
The NRG2 protein plays a significant role in the PI3K/AKT signaling and MAPK/ERK pathways. These pathways are essential for regulating cell survival proliferation and differentiation. Through the ErbB receptors NRG2 influences these signaling pathways intimately connecting it with other proteins such as AKT1 in the PI3K/AKT pathway and MEK1/2 in the MAPK/ERK pathway which further amplify its regulatory roles in cellular processes.
NRG2 is implicated in schizophrenia and peripheral nerve diseases. The interaction of NRG2 with the ErbB4 receptor has associations with schizophrenia where alterations in this signaling pathway may affect cognitive functions. In peripheral nerve diseases NRG2's role in neuron repair and maintenance can be significant linking it to ErbB3 in supporting nerve regeneration processes which becomes vital when addressing neuropathies.